ROKEPIE effectively promotes cell viability of primary human hematopoietic stem cells during hypothermic storage. Center for Stemm Cell R&D, Hacettepe University, Ankara Click here for the complete poster
We like to share our encouraging results; When ROKEPIE® is used at 1/10 dilution and dispensed in both apical and basal wells of the plate, we see a major improvement. Without adding ROKEPIE® we simply cannot store and ship in cells in our gel formulation.
We have tested ROKEPIE® in a 1:20 dilution added to our cell culture by storing them in the fridge for 24 hrs. The morphology after storage was very good, many mitoses were visible. The 3D model images after 10 and 14 days were very good, as well as the barrier function.
We tested the ROKEPIE® with primary human keratinocytes, for 3 days at 4°C. We found that the cells recommenced normal proliferation after they were returned to the incubator. It is of interest for transport purposes, which we will investigate further.
Thus far, I am quite satisfied with the results that we have been achieving using the ROKEPIE® hibernation reagent (especially also given the ease of its use) and I’d be happy to perform a few more experiments.
Are you using ROKEPIE? Please let us know your experiences so we can improve our product!