Hypothermic preservation 3.0 – no need for medium changes, just Add & Use ROKEPIE®.

In terms of cell biopreservation, two different approaches are used: Cryogenic preservation in sub-zero temperatures and hypothermic preservation in the range of 1-35 °C. They are both used in order to store, transport of ship cells for a specific period of time. Choosing the right preservation method depends on a lot of variables, with the need for short- or long term storage as one of the most important questions. If long-term preservation is not requested, avoiding cryopreservation will bring many benefits. A good alternative can be found in hypothermic preservation – the storage of cells in cold conditions (2-8°C).

Short-term preservation The benefits of using a hypothermic method over cryogenic storage for short-term preservation are evident. Being able to omit out the use of dimethyl sulfoxide (DMSO), which is toxic to cells at ambient temperatures, decreases the risk of cell loss, decreased cell differentiation or adverse reactions from patients. The protocols for hypothermic preservation are less complicated, thus saving costs and labor. Even so, most current hypothermic preservation solutions still require a medium change before and after cool storage. Their process protocols still include these extra steps, possibly leading to unwanted cell stress or even cell damage.

What makes ROKEPIE® unique compared to other hypothermic preservation solutions? The ROKEPIE® additive is a perfect alternative for cryopreservation in short-term storage situations, being an hypothermic preservation method. But it is unique with regard to its competitors. The unique feature of ROKEPIE® is that it is a non-toxic additive. This means that you can simply add it before cooling and storing, there is no need to remove the medium. And there is also no need to remove medium before rewarming. It is non-toxic when it is brought to 37°C and ROKEPIE® supports cell proliferation after rewarming!

  • Easy to use thanks to the Add & Use principle – less complicated protocols, thus time saving
  • It’s an additive – no need for medium change
  • It’s non-toxic – can be rewarmed up to 37°C
  • It’s fully synthetic – no animal component
  • It even supports cell growth after rewarming – non-toxic

Why use it?